Dermal-epidermal membrane systems by using human keratinocytes and mesenchymal stem cells isolated from dermis

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• 作者：Simona Salerno^a ; ^{s.salerno@itm.cnr.it} ; Antonietta Messina^a ; Francesca Giordano^b ; Augustinus Bader^c ; Enrico Drioli^a ; ^d ; Loredana De Bartolo^a ; ^{l.debartolo@itm.cnr.it} ; ^{loredana.debartolo@cnr.it}
• 关键词：Biomaterials ; Mesenchymal stem cells ; Dermal-epidermal substitutes ; Membranes ; Chitosan ; Polycaprolactone ; Skin tissue engineering
• 刊名：Materials Science and Engineering: C
• 出版年：2017
• 出版时间：1 February 2017
• 年：2017
• 卷：71
• 期：Complete
• 页码：943-953
• 全文大小：2940 K
• 卷排序：71

文摘

Dermal-epidermal membrane systems were developed by co-culturing human keratinocytes with Skin derived Stem Cells (SSCs), which are Mesenchymal Stem Cells (MSCs) isolated from dermis, on biodegradable membranes of chitosan (CHT), polycaprolactone (PCL) and a polymeric blend of CHT and PCL. The membranes display physico-chemical, morphological, mechanical and biodegradation properties that could satisfy and fulfil specific requirements in skin tissue engineering. CHT membrane exhibits an optimal biodegradation rate for acute wounds; CHT-PCL for the chronic ones. On the other hand, PCL membrane in spite of its very slow biodegradation rate exhibits mechanical properties similar to in vivo dermis, a lower hydrophilic character, and a surface roughness, all properties that make it able to sustain cell adhesion and proliferation for in vitro skin models. Both CHT–PCL and PCL membranes guided epidermal and dermal differentiation of SSCs as pointed out by the expression of cytokeratins and the deposition of the ECM protein fibronectin, respectively. In the dermal-epidermal membrane systems, a more suitable microenvironment for the SSCs differentiation was promoted by the interactions and the mutual interplay with keratinocytes. Being skin tissue-biased stem cells committed to their specific final dermal and/or epidermal cell differentiation, SSCs are more suitable for skin tissue engineering than other adult MSCs with different origin. For this reason, they represent a useful autologous cell source for engineering skin substitutes for both in vivo and in vitro applications.