Differentially regulated genes in Solanum tuberosum in response to ¡°Mexican potato purple top phytoplasma¡± infection

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• 作者：Rosa Marí ; a Longoria-Espinoza^a ; ¹ ; Nadia Rubí ; Douriet-Gá ; mez^b ; ² ; Melina Ló ; pez-Meyer^b ; ² ; Francisco Quiroz-Figueroa^b ; ² ; Mario Bueno-Ibarra^b ; ² ; Jesú ; s M&#233 ; ndez-Lozano^b ; ² ; Marí ; a Elena Santos-Cervantes^b ; ² ; Rub&#233 ; n F&#233 ; lix-Gast&#233 ; lum^a ; ¹ ; Jesú ; s Alicia Chá ; vez-Medina^b ; ² ; Norma Elena Leyva-Ló ; pez^b ; ^{neleyval@ipn.mx}
• 关键词：Solanum tuberosum L. ; PPT disease ; Phytoplasma&ndash ; plant interaction ; Differentially expressed genes ; SSH ; Macroarray screening ; Real-time PCR
• 刊名：Physiological and Molecular Plant Pathology
• 出版年：2013
• 出版时间：January, 2013
• 年：2013
• 卷：81
• 期：Complete
• 页码：33-44
• 全文大小：894 K

文摘

Potato plants infected by phytoplasmas exhibit an array of symptoms that suggest interference within the normal balance of plant metabolism. Suppression subtractive hybridization (SSH), macroarray screening and real-time PCR were performed to identify differentially expressed genes in phytoplasma-infected in?vitro potato plants. Gene expression analysis revealed the up-regulation of genes involved in several metabolic pathways, including ERF, WRKY, HSP90, SAP6, AN1-like ZFP, and an unknown protein; the down-regulation of genes encoding monodehydroascorbate reductase and a putative membrane protein was also observed. The results presented here provide the groundwork for addressing new questions to better understand the phytoplasma-plant interaction.