The 13C abundance in the amino acid fraction of blood in goats approached to the 13C abundance in C3 or C4 diet with the feeding time extension, and it reached a stable level after labeled for 12 weeks.
The true protein digestibility in the forestomach, whole intestine and whole digestive tract for goats fed C4 diet tended to be higher than for those fed C3 diet.
The goat tissue protein could be stable labeled with 13C naturally enriched in the diet, and 13C as natural isotope marker can be used to determine true amino acid digestibility.