Differentiation and characterization of human pluripotent stem cell-derived brain microvascular endothelial cells
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- 作者：Matthew J. Stebbins ; Hannah K. Wilson ; Scott G. Canfield ; Tongcheng Qian ; Sean P. Palecek ; sppalecek@wisc.edu" class="auth_mail" title="E-mail the corresponding author ; Eric V. Shusta ; eshusta@wisc.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：BBB ; blood&ndash ; brain barrier ; CNS ; central nervous system ; hPSCs ; human pluripotent stem cells ; BMECs ; brain microvascular endothelial cells ; P-gp ; P-glycoprotein ; RA ; retinoic acid ; UM ; unconditioned medium ; EC ; endothelial cell ; EC ; + ; RA medium ; EC medium supplemented with RA ; TEER ; transendothelial electrical resistance ; NEAA ; nonessential amino acids ; KOSR ; knockout serum replacement ; hESFM ; human endothelial serum-free medium ; PDS ; platelet poor plasma-derived serum ; bFGF ; basic
- 刊名：Methods
- 出版年：2016
- 出版时间：15 May 2016
- 年：2016
- 卷：101
- 期：Complete
- 页码：93-102
- 全文大小：1679 K
文摘
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Human pluripotent stem cells differentiate to blood-brain barrier endothelial cells.
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hPSC-derived brain endothelial cells exhibit BBB phenotypes.
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hPSCs provide a scalable and renewable cell source for modeling the human BBB.