Détermination de l'hémoglobine plasmatique et évaluation spectrophotométrique de lhemolyse en biochimie clinique
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- 作者：Hicham Mezzou ; Anis Belhaj Khelifa ; Fadoua Neffati ; Wahiba Douki ; Adel Ben Amor ; Mohamed Fadhel Najjar
- 关键词：Haemoglobin ; determination ; haemolysis ; estimation
Author Keywords ; Hé ; moglobine ; dosage ; hé ; molyse ; é ; valuation - 刊名：Revue Francophone des Laboratoires
- 出版年：2006
- 出版时间：November 2006
- 年：2006
- 卷：2006
- 期：386
- 页码：59-64
- 全文大小：3112 K
文摘
The measurement of plasma haemoglobin is utilized in the evaluation of various haemolytic phenomenons, especially at the time of sampling. Besides, the methods used to titrate plasmatic haemoglobin, an analysis which is of great interest to cardiac surgery, must be very accurate, because plasmatic levels are much lower than those found in whole blood. The purpose of this report is to illustrate the advantages of a direct spectrophotometric scanning technique for determination of plasma haemoglobin.
The scanning method demonstrates linearity, precision; and sensitivity suitable for clinical use and not affected by the presence of Bilirubin, nor triglycerides.
The correlation was assessed with a spectrophotometric method using the 3,3;5,5′-tetramethylbenzidine (TMB), a non carcinogenic reagent. The reaction of haemoglobin with TMB was studied with a Hitachi U-2000 UV-Visible spectrometer. The method was precise (CV < 1,00 % ), accurate (CE < 5,00 % ) and showed excellent recovery of haemolysis (R > 0,95). The CV for the, manual assay was less than 1 % .