Detection of protective antigen, an anthrax specific toxin in human serum by using surface plasmon resonance
详细信息 查看全文
- 作者：Neha Ghosh ; Nidhi Gupta ; Garima Gupta ; Mannan Boopathi ; Vijay Pal ; Ajay Kumar Goel
- 关键词：Anthrax ; Bacillus anthracis ; Monoclonal antibody ; Protective antigen ; Surface plasmon resonance
- 刊名：Diagnostic Microbiology and Infectious Disease
- 出版年：2013
- 出版时间：September, 2013
- 年：2013
- 卷：77
- 期：1
- 页码：14-19
- 全文大小：1090 K
文摘
In this study, surface plasmon resonance (SPR) technology was used for the sensitive detection of protective antigen (PA), an anthrax specific toxin in spiked human serum samples. A monoclonal antibody raised against Bacillus anthracis PA was immobilized on carboxymethyldextran-modified gold chip, and its interaction with PA was characterized in situ by SPR. By using kinetic evaluation software, KD (equilibrium constant) and Bmax (maximum binding capacity of analyte) were found to be 20 fM and 18.74 m¡ã, respectively. The change in Gibb's free energy (?G= ?78.04 kJ/mol) confirmed the spontaneous interaction between antigen and antibody. The assay could detect 1 pg/mL purified PA. In PA-spiked human serum samples, 10 pg/mL of PA could be detected. Presence of PA in blood samples serves as an important early diagnostic marker for B. anthracis infections. Thus, SPR test can be a sensitive assay for detection of anthrax at early stages of infection.