Exoproteome analysis of a novel strain of Bacillus cereus implicated in disease resembling cutaneous anthrax

详细信息    查看全文

• 作者：Neha Ghosh ^{nehughosh26@gmail.com" class="auth_mail} ; Ajay Kumar Goel ^{akgoel73@yahoo.co.uk" class="auth_mail} ; Syed Imteyaz Alam ; ^{syimteyaz@gmail.com" class="auth_mail}
• 关键词：Bacillus cereus ; Cutaneous anthrax ; S-layer protein ; Sphingomyelinase
• 刊名：Infection, Genetics and Evolution
• 出版年：March, 2014
• 年：2014
• 卷：22
• 期：Complete
• 页码：1-11
• 全文大小：1422 K

文摘

Bacillus cereus belongs to B. cereus sensu lato group, shared by six other related species including Bacillus anthracis. B. anthracis is the causative agent for serious illness affecting a wide range of animals as well as humans and is a category A Biological and Toxin Warfare (BTW) agent. Recent studies indicate that a Bacillus species other than B. anthracis can cause anthrax-like disease and role of anthrax virulence plasmids (pXO1 and pXO2) on the pathogenicity of B. cereus has been documented. B. cereus strain TF5 was isolated from the tissue fluid of cutaneous anthrax-like skin lesions of a human patient from an anthrax endemic area in India. The strain harboured a PA gene, however, presence of pXO1 or pXO2-like plasmids could not be ascertained using reported primers. Abundant exoproteome of the strain in the early stationary phase was elucidated using a 2-DE MS approach and compared with that from a reference B. cereus strain. Analysis of proteins showing qualitative and quantitative differences between the two strains indicated an altered regulatory mechanism and putative role of S-layer protein and sphingomyelinase in the pathogenesis of strain TF5. Phylogenetic analysis of the S-layer protein indicated close affiliation of the strain with anthracis-like B. cereus strains such as B. cereus var. anthracis strain CI; whereas sphingomyelinase exhibited specific relationship with all the strains of B. anthracis apart from that with anthracis-like B. cereus strains.