Rapid and Sensitive Chemiluminescent Enzyme Immunoassay for the Determination of Neomycin Residues in Milk

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• 作者：Peng Jie LUO^aAuthor Vitae ; Jian Bo ZHANG^aAuthor Vitae ; Hua Li WANG^a ; Xia CHEN^b ; Nan WU^c ; Yun Feng ZHAO^a ; Xiao Mei WANG^c ; Hong ZHANG^a ; Ji Yue ZHANG^a ; Lei ZHU^a ; Wen Xiao JIANG^c ; ^{jiangwenxiao@szu.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 刊名：Biomedical and Environmental Sciences
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：29
• 期：5
• 页码：374-378
• 全文大小：848 K

文摘

Immunoassays greatly contribute to veterinary drug residue analysis. However, there are few reports on detecting neomycin residues by immunoassay. Here, a rapid and sensitive chemiluminescent enzyme immunoassay (CLIEA) was successfully developed for neomycin residue analysis. CLIEA demonstrated good cross-reactivity for neomycin, and the IC₅₀ value was 2.4 ng/mL in buffer. The average recovery range was 88.5%–105.4% for spiked samples (10, 50, and 100 μg/kg), and the coefficient of variation was in the range of 7.5%–14.5%. The limit of detection of CLEIA was 9.4 μg/kg, and this method was compared with the liquid chromatography-tandem mass spectrometry method using naturally contaminated samples, producing a correlation coefficient of >0.95. We demonstrate a reliable CLIEA for the rapid screening of neomycin in milk.