Effect of cell seeding on neotissue formation in a tissue engineered trachea

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• 作者：Elizabeth S. Clark^a ; ^b ; Cameron Best^a ; Ekene Onwuka^a ; ^c ; Tadahisa Sugiura^a ; Nathan Mahler^a ; Brad Bolon^b ; ^d ; Andrew Niehaus^e ; Iyore James^a ; Narutoshi Hibino^a ; ^f ; Toshiharu Shinoka^a ; ^f ; Jed Johnson^g ; Christopher K. Breuer^a ; ^h ; ^{Christopher.Breuer@NationwideChildrens.org" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Tissue engineering ; Trachea ; Electrospinning ; Bone marrow mononuclear cells ; Vacuum seeding ; Large animal model
• 刊名：Journal of Pediatric Surgery
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：51
• 期：1
• 页码：49-55
• 全文大小：1263 K

文摘

Surgical management of long segment tracheal disease is limited by a paucity of donor tissue and poor performance of synthetic materials. A potential solution is the development of a tissue-engineered tracheal graft (TETG) which promises an autologous airway conduit with growth capacity.

Methods

We created a TETG by vacuum seeding bone marrow-derived mononuclear cells (BM-MNCs) on a polymeric nanofiber scaffold. First, we evaluated the role of scaffold porosity on cell seeding efficiency in vitro. We then determined the effect of cell seeding on graft performance in vivo using an ovine model.

Results

Seeding efficiency of normal porosity (NP) grafts was significantly increased when compared to high porosity (HP) grafts (NP: 360.3 ± 69.19 × 10³ cells/mm²; HP: 133.7 ± 22.73 × 10³ cells/mm²; p < 0.004). Lambs received unseeded (n = 2) or seeded (n = 3) NP scaffolds as tracheal interposition grafts for 6 weeks. Three animals were terminated early owing to respiratory complications (n = 2 unseeded, n = 1 seeded). Seeded TETG explants demonstrated wound healing, epithelial migration, and delayed stenosis when compared to their unseeded counterparts.

Conclusion

Vacuum seeding BM-MNCs on nanofiber scaffolds for immediate implantation as tracheal interposition grafts is a viable approach to generate TETGs, but further preclinical research is warranted before advocating this technology for clinical application.