Clinical significance of immunohistochemically detected extracellular matrix proteins and their spatial distribution in primary cancer

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• 作者：B. Čunderlí ; ková ; ^a ; ^b ; ^{beata@ilc.sk" class="auth_mail" title="E-mail the corresponding author}
• 关键词：2D ; two-dimensional ; 3D ; three-dimensional ; APC ; adenomatous polyposis coli ; BCC ; basal cell carcinoma ; BM ; basement membrane ; CEA ; carcinoembryonic antigen ; CIS ; carcinoma in situ ; CNS ; central nervous system ; ECM ; extracellular matrix ; IHC ; immunohistochemical ; LOX ; lysyl oxidase ; MMP ; matrix metalloproteinase ; NSCLC ; non-small cell lung carcinoma ; PCNA ; proliferating cell nuclear antigen ; SCC ; squamous cell carcinoma ; SCLC ; small cell lung carcinoma ; SHG ; second harmonic generation ; SPARC ; se
• 刊名：Critical Reviews in Oncology and Hematology
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：105
• 期：Complete
• 页码：127-144
• 全文大小：2259 K

文摘

Our understanding of cancer has evolved mainly from results of studies utilizing experimental models. Simplification inherent to in vitro cell culture models enabled potential ways of cell behaviour in response to various external stimuli to be described, but it has led also to disappointments in clinical trials, presumably due to the lack of crucial tissue components, including extracellular matrix (ECM). ECM and its role in healthy and diseased tissues are being explored extensively and significance of ECM for cell behaviour has been evidenced experimentally. Part of the information gathered in such research that is relevant for natural conditions of a human body can be identified by carefully designed analyses of human tissue samples. This review summarizes published information on clinical significance of ECM in cancer and examines whether effects of ECM on cell behaviour evidenced in vitro, could be supported by clinically based data acquired from analysis of tissue samples. Based on current approaches of clinical immunohistochemical analyses, impact of ECM components on tumour cell behaviour is vague. Except of traditionally considered limitations, other reasons may include lack of stratification of analyzed cases based on clinicopathologic parameters, inclusion of patients treated postoperatively by different treatments or neglecting complexity of interactions among tumour constituents. Nevertheless, reliable immunohistochemical studies represent a source of crucial information for design of tumour models comprising ECM corresponding to real clinical situation. Knowledge gathered from such immunohistochemical studies combined with achievements in tissue engineering hold promise for reversal of the unfavourable trends in the current translational oncologic research.