Long-Term High-Resolution Imaging of Developing C. elegans Larvae with Microfluidics

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• 作者：Wolfgang Keil¹ ; ² ; ³ ; ^{wkeil@rockefeller.edu} ; Lena M. Kutscher² ; Shai Shaham² ; ^{shaham@rockefeller.edu} ; Eric D. Siggia¹ ; ^{siggiae@rockefeller.edu}
• 关键词：Caenorhabditis elegans ; microfluidics ; long-term imaging ; larval development ; vulval development ; linker cell death ; PDA neuron ; transdifferentiation ; PVD neuron ; dendritic arborization
• 刊名：Developmental Cell
• 出版年：2017
• 出版时间：23 January 2017
• 年：2017
• 卷：40
• 期：2
• 页码：202-214
• 全文大小：5443 K
• 卷排序：40

文摘

Multichannel time-lapse imaging of C. elegans larvae of any stage for up to 72 hr Cell-cycle timing statistics of vulval lineages for >100 animals Fluorescent reporter expression during divisions, differentiation, and cell death Automated image registration enables visualizing complex neurite outgrowth