The gain in brain: novel imaging techniques and multiplexed proteomic imaging of brain tissue ultrastructure

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• 作者：Kristina D Micheva¹ ; ^{kmicheva@stanford.edu} ; Marcel P Bruchez² ; ³ ; ⁴ ; ^{bruchez@cmu.edu}
• 刊名：Current Opinion in Neurobiology
• 出版年：2012
• 出版时间：February, 2012
• 年：2012
• 卷：22
• 期：1
• 页码：94-100
• 全文大小：1139 K

文摘

The rapid accumulation of neuroproteomics data in recent years has prompted the emergence of novel antibody-based imaging methods that aim to understand the anatomical and functional context of the multitude of identified proteins. The pioneering field of ultrastructural multiplexed proteomic imaging now includes a number of high resolution methods, such as array tomography, stimulated emission depletion microscopy, stochastic optical reconstruction microscopy and automated transmission electron microscopy, which allow a detailed molecular characterization of individual synapses and subsynaptic structures within brain tissues for the first time. While all of these methods still face considerable limitations, a combined complementary approach building on the respective strengths of each method is possible and will enable fascinating research into the proteomic diversity of the nervous system.