Adenovirus 36 antibody detection: Improving the standard serum neutralization assay

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• 作者：Cynthia L. Chappell^a ; ^c ; ^{Cynthia.L.Chappell@uth.tmc.edu} ; Mary Dickerson^d ; ³ ; ^{mdickerson@ocm.utah.edu} ; R. Sue Day^b ; ^c ; ¹ ; ^{Rena.S.Day@uth.tmc.edu} ; Olga Dubuisson^e ; ² ; ^{Olga.Dubuisson@pbrc.edu} ; Nikhil V. Dhurandhar^e ; ⁴ ; ^{Nikhil.dhurandhar@ttu.edu}
• 关键词：Serological assay ; Immunochemical staining ; Elisa ; Obesity ; Adiposity ; Infectobesity
• 刊名：Journal of Virological Methods
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：239
• 期：Complete
• 页码：69-74
• 全文大小：1124 K
• 卷排序：239

文摘

Adenovirus 36 is linked to human obesity but evidence for causation is needed. The serum neutralization assay for specific antibody is the lengthy and laborious. An ICC assay is more objective and detects virus in half the incubation time. Sensitivity and specificity of ICC-tested sera vs SNA were 80.0% and 95.7%. The more rapid and objective ICC assay will make large population studies feasible.