Targeting synthetic Human Papillomavirus (HPV) L2 disulfide-induced N-terminus conformational epitopes for pan-HPV vaccine development

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• 作者：Sujita Khanal^a ; ^b ; ¹ ; ^{sujita.khanal@louisville.edu" class="auth_mail" title="E-mail the corresponding author} ; Eric Daniel Ferraris^b ; ^d ; ¹ ; ^{ericdferraris@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Maryam Zahin^b ; ^{m0zahi01@louisville.edu" class="auth_mail" title="E-mail the corresponding author} ; Joongho Joh^b ; ^c ; ^{j0joh001@louisville.edu" class="auth_mail" title="E-mail the corresponding author} ; Shin-je Ghim^b ; ^c ; ^{sjghim01@louisville.edu" class="auth_mail" title="E-mail the corresponding author} ; Alfred Bennett Jenson^b ; ^{abjens01@louisville.edu" class="auth_mail" title="E-mail the corresponding author} ; ^{abjenson@yahoo.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：HPV ; Human Papillomavirus ; ELISA ; enzyme-linked immunosorbent assay
• 刊名：Experimental and Molecular Pathology
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：99
• 期：2
• 页码：330-334
• 全文大小：1137 K

文摘

Current vaccines against Human Papillomavirus (HPV) are highly effective and based on recombinant virus-like particles (VLPs) of the major capsid protein L1. Since these vaccines are HPV type-specific and expensive for global implementation, an alternative, broader-spectrum immunogen would be the N-terminus of the minor capsid protein L2 that induces low titered broadly cross-neutralizing antibodies. Here we analyzed the reactivity of different synthetic L2 peptides containing N-terminus amino acids 17–36 in order to test their antigenicity.

Methods

Different synthetic peptides were designed to target the 17–36 amino acid sequences, present in highly antigenic amino-terminus of L2 protein. Six different peptides including Cys22–Cys28 disulfide bonded cyclized L2 peptide were examined for their antigenicity against mouse monoclonal antibody RG-1 and rabbit polyclonal antisera to HPV L2 by enzyme-linked immunosorbent assay (ELISA).

Results

Here we report that the cyclized form of synthetic L2 peptide, which is formed through Cys22–Cys28 disulfide bridges, has the highest reactivity to antibodies than other synthetic L2 peptides.

Conclusion

A cyclized L2 peptide has potential to be an excellent candidate to formulate a low-cost, broadly protective pan-oncogenic HPV vaccine.