High concentrations of myeloperoxidase in the equine uterus as an indicator of endometritis

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• 作者：Sonia Parrilla-Hernandez^a ; ^c ; ^{sphvet@gmail.com" class="auth_mail} ; Jé ; rô ; me Ponthier^a ; ^c ; Thierry Y. Franck^b ; Didier D. Serteyn^b ; ^c ; Sté ; fan C. Deleuze^a ; ^c
• 关键词：Mare ; Endometritis ; Myeloperoxidase ; Intraluminal fluid ; Hyperedema ; Cytology
• 刊名：Theriogenology
• 出版年：15 April, 2014
• 年：2014
• 卷：81
• 期：7
• 页码：936-940
• 全文大小：216 K

文摘

Intraluminal fluid and excessive abnormal hyperedema are regularly used for the diagnosis of endometritis in the mare, which is routinely confirmed by the presence of neutrophils on endometrial smears. Studies show a relation between neutrophils and myeloperoxidase (MPO), an enzyme contained in and released by neutrophils during degranulation or after cell lysis. This enzyme has been found in many fluids and tissues, and associated with different inflammatory pathologies in the horse. The aims of this study were to assess the presence and concentration of MPO in the equine uterus, and to investigate its relation with neutrophils, and other clinical signs of endometritis. Mares (n = 51) were evaluated for the presence of intraluminal fluid and excessive endometrial edema before breeding, and a small volume lavage and cytology samples were obtained. From 69 cycles, supernatant of the uterine flushes was analyzed with a specific equine MPO ELISA assay to measure MPO concentration. Cytology samples were used for the diagnosis of endometritis. Myeloperoxidase was present in the uterus of all estrus mares in highly variable concentrations. Myeloperoxidase concentrations were significantly (P < 0.05) higher in samples with positive cytologies and in the presence of intraluminal fluid. Occasionally, some samples with negative cytologies showed high MPO concentration, but the opposite was never observed. Cycles presenting hyperedema weren't associated with high concentration of MPO, intraluminal fluid, or positive cytology, making it a poor diagnostic tool of endometritis.