Identification of dairy lactic acid bacteria by tRNA^Ala-23S rDNA-RFLP

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• 作者：Andrea Mancini ; Camilla Lazzi ; Valentina Bernini ; Erasmo Neviani ; Monica Gatti ; ^{monica.gatti@unipr.it}
• 关键词：Banding pattern-based method ; Methods comparison ; Fingerprinting ; Dairy isolates ; P1 RAPD ; (GTG)5 rep-PCR
• 刊名：Journal of Microbiological Methods
• 出版年：2012
• 出版时间：December, 2012
• 年：2012
• 卷：91
• 期：3
• 页码：380-390
• 全文大小：1897 K

文摘

The aim of this study was to evaluate the potential of target tRNA^Ala-23S ribosomal DNA for identification of lactic acid bacteria strains associated with dairy ecosystem. For this purpose tRNA^Ala-23S ribosomal DNA Restriction Fragment Length Polymorphism (tRNA^Ala-23S rDNA-RFLP) was compared with two widely used DNA fingerprinting methods - P1 Random Amplified Polymorphic DNA (RAPD), (GTG)5 repetitive extragenic palindromic PCR (rep-PCR) - for their ability to identify different species on a set of 10 type and 34 reference strains.

Moreover, 75 unknown isolates collected during different stages of Grana Padano cheese production and ripening were identified using tRNA^Ala-23S rDNA-RFLP and compared to the RFLP profiles of the strains in the reference database. This study demonstrated that the target tRNA^Ala-23S rDNA has high potential in bacterial identification and tRNA^Ala-23S rDNA-RFLP is a promising method for reliable species-level identification of lactic acid bacteria (LAB) in dairy products.