NF-¦ÊB activity is inversely correlated to RNF11 expression in Parkinson's disease

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• 作者：Elaine Pranski^a ; ^c ; ^{epransk@emory.edu} ; Carson D. Van Sanford^a ; ^c ; ^{cvansan2@gmail.com} ; Nirjari Dalal^a ; ^c ; ^{nvdalal@emory.edu} ; Adam L. Orr^a ; ^c ; ^{aorr25@gmail.com} ; Dipan Karmali^a ; ^c ; ^{karmali.dipan@gmail.com} ; Deborah S. Cooper^a ; ^c ; ^{dscoope@emory.edu} ; Marla Gearing^b ; ^c ; ^{mgearin@emory.edu} ; James J. Lah^a ; ^c ; ^{jlah@emory.edu} ; Allan I. Levey^a ; ^c ; ^{alevey@emory.edu} ; Ranjita Betarbet^a ; ^c ; ^{rbetarb@emory.edu}
• 关键词：RNF11-RING ; finger protein 11 ; NF-¦ÊB ; nuclear factor kappa-light-chain-enhancer of activated B cells ; PD ; Parkinson's disease ; SN ; substantia nigra ; DAB ; diaminobenzedine
• 刊名：Neuroscience Letters
• 出版年：2013
• 出版时间：28 June, 2013
• 年：2013
• 卷：547
• 期：Complete
• 页码：16-20
• 全文大小：1254 K

文摘

RING finger protein 11 (RNF11), a negative regulator of NF-¦ÊB signaling pathway, colocalizes with ¦Á-synuclein and is sequestered in Lewy bodies in Parkinson's disease (PD). Since persistent NF-¦ÊB activation is reported in PD, in this report we investigated if RNF11 expression level is correlated to activated NF-¦ÊB in PD. We examined RNF11 expression levels in correlation to phospho-p65, a marker for activated NF-¦ÊB, in control and PD brain tissue from cerebral cortex. In addition we performed double immunofluorescence labeling experiments to confirm this correlation. Our investigations demonstrated that the neuronal RNF11 expression was down-regulated in PD and was usually associated with increased expression of phospho-p65. Double labeling confirmed that loss of neuronal RNF11 was linked to increased phospho-p65 expression, suggesting that persistent presence of NF-¦ÊB activation could be due to decreased levels of its negative regulator. Our data exemplifies the relevance of RNF11 and persistent NF-¦ÊB activation in PD.