Allele-specific TF complex assembly and disruption on the non-coding variant is based on binding affinity and dissociation rate.
NF-KBp50, p65 and HIF1a preferentially bind the C-allele of rs7901656 in FAS.
NF-KBp50, p65 and HIF1a form higher order heteromultimeric complexes with synergism in binding their motifs around rs7901656.
p65 and HIF1a binding are considerably influenced by flanking sequences around their motifs while NF-KBp50 is not.
TF disruption on rs7901656 is associated with cystic fibrosis disease outcome.