Role of nuclear factor of activated T-cells 5 in regulating hypertonic-mediated secretin receptor expression in kidney collecting duct cells
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- 作者：Oscar W.H. Chuaa ; Kenneth K.L. Wonga ; Ben C. Kob ; Sookja K. Chungc ; d ; Billy K.C. Chowa ; Leo T.O. Leee ; LTOLee@umac.mo" class="auth_mail" title="E-mail the corresponding author
- 关键词：cAMP ; cyclic adenosine 3&prime ; 5&prime ; -monophosphate ; ChIP ; chromatin immunoprecipitation ; NFAT5 ; nuclear factor of activated T-cells 5 ; ORE ; osmotic response element ; SCTR ; secretin receptor ; siRNA ; small interfering RNA
- 刊名：Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：1859
- 期：7
- 页码：922-932
- 全文大小：1579 K
文摘
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Hypertonicity induces the expression of NFAT5 and SCTR in kidney collecting duct cell lines.
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NFAT5 siRNA abolishes the hypertonic-induced SCTR promoter activity and SCTR expression.
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ChIP assay demonstrated the binding of NFAT5 to the SCTR promoter region − 367/− 228.
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The role of NFAT5 in mediating hypertonic-induced SCTR expression was suggested by in vivo studies with NFAT5+/− mice.