Expression, fermentation and purification of a predicted intrinsically disordered region of the transcription factor, NFAT5
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- 作者：Jenna F. DuMonda ; jenna.dumond@nih.gov" class="auth_mail" title="E-mail the corresponding author ; Yi Heb ; hey4@nhlbi.nih.gov" class="auth_mail" title="E-mail the corresponding author ; Maurice B. Burga ; burgm@mail.nih.gov" class="auth_mail" title="E-mail the corresponding author ; Joan D. Ferrarisa ; ferraris@nhlbi.nih.gov" class="auth_mail" title="E-mail the corresponding author
- 关键词：Intrinsically disordered proteins ; Transcription factors ; Hypertonicity ; Fermentation
- 刊名：Protein Expression and Purification
- 出版年：2015
- 出版时间：November 2015
- 年：2015
- 卷：115
- 期：Complete
- 页码：141-145
- 全文大小：857 K
文摘
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Construct pET-MBP-872-1271 was expressed in E. coli and grown through fermentation.
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Specific AcTEV cleavage followed by His-tag affinity further purified 872-1271.
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Continued purification with a DEAE and HIC columns completed purification of 872-1271.
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Concentrated to 0.2 mg/mL; sufficient to study intrinsic disorder using fluorescence.
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Protein mass confirmed with HPLC–MS.