Multifunctional particles for melanoma-targeted drug delivery
详细信息 查看全文
- 作者：Aniket S. Wadajkar ; Zarna Bhavsar ; Cheng-Yu Ko ; Bhanuprasanth Koppolu ; Weina Cui ; Liping Tang ; Kytai T. Nguyen
- 关键词：Biodegradable polymers ; Magnetite nanoparticles ; Thermo-responsive polymers ; Targeted drug delivery ; Melanoma
- 刊名：Acta Biomaterialia
- 出版年：2012
- 出版时间：August, 2012
- 年：2012
- 卷：8
- 期：8
- 页码：2996-3004
- 全文大小：1198 K
文摘
New magnetic-based core-shell particles (MBCSPs) were developed to target skin cancer cells while delivering chemotherapeutic drugs in a controlled fashion. MBCSPs consist of a thermo-responsive shell of poly(N-isopropylacrylamide-acrylamide-allylamine) and a core of poly(lactic-co-glycolic acid) (PLGA) embedded with magnetite nanoparticles. To target melanoma cancer cells, MBCSPs were conjugated with Gly-Arg-Gly-Asp-Ser (GRGDS) peptides that specifically bind to the receptors of melanoma cells. MBCSPs consist of unique multifunctional and controlled drug delivery characteristics. Specially, they can provide dual drug release mechanisms (a sustained release of drugs through degradation of PLGA core and a controlled release in response to changes in temperature via thermo-responsive polymer shell), and dual targeting mechanisms (magnetic localization and receptor-mediated targeting). Results from in vitro studies indicate that GRGDS-conjugated MBCSPs have an average diameter of 296 nm and exhibit no cytotoxicity towards human dermal fibroblasts up to 500 ¦Ìg ml?1. Further, a sustained release of curcumin from the core and a temperature-dependent release of doxorubicin from the shell of MBCSPs were observed. The particles also produced a dark contrast signal in magnetic resonance imaging. Finally, the particles were accumulated at the tumor site in a B16F10 melanoma orthotopic mouse model, especially in the presence of a magnet. Results indicate great potential of MBCSPs as a platform technology to target, treat and monitor melanoma for targeted drug delivery to reduce side effects of chemotherapeutic reagents.