Recombinant ELISA using baculovirus-expressed VP2 for detection of antibodies against canine parvovirus
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- 作者：Gabriella Elia ; Costantina Desario ; Giulia Pezzoni ; Michele Camero ; Emiliana Brocchi ; Nicola Decaro ; Vito Martella ; Canio Buonavoglia
- 关键词：CPV-2 ; Vaccination ; Maternally derived antibodies ; ELISA ; Recombinant antigen
- 刊名：Journal of Virological Methods
- 出版年：2012
- 出版时间：September, 2012
- 年：2012
- 卷：184
- 期：1-2
- 页码：98-102
- 全文大小：255 K
文摘
The gene encoding the VP2 protein of canine parvovirus type 2 was expressed in an insect-baculovirus system. The recombinant (r) VP2 was similar antigenically/functionally to the native capsid protein as demonstrated by hemagglutination, Western blotting and hemagglutination inhibition test, using Canine parvovirus type-2 (CPV-2) positive sera. An enzyme-linked immunosorbent assay (ELISA) using the rVP2 was used for testing CPV-2 positive and negative sera from dogs and for determining the threshold of maternally derived antibodies interfering with successful vaccination of pups against CPV-2.