β-caryophyllene, a dietary cannabinoid, complexed with β-cyclodextrin produced anti-hyperalgesic effect involving the inhibition of Fos expression in superficial dorsal horn

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• 作者：Lucindo J. Quintans-Jú ; nior^a ; ^c ; ¹ ; ^{lucindojr@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; ^{lucindo@pq.cnpq.br" class="auth_mail" title="E-mail the corresponding author} ; Adriano A.S. Araú ; jo^b ; ¹ ; Renan G. Brito^a ; ^c ; Priscila L. Santos^a ; Jullyana S.S. Quintans^a ; Paula P. Menezes^b ; Mairim R. Serafini^b ; Gabriel F. Silva^c ; Flavio M.S. Carvalho^d ; Nicole K. Brogden^e ; Kathleen A. Sluka^f
• 关键词：β-Caryophyllene ; β-Cyclodextrin ; Chronic pain ; Pain ; Hyperalgesia ; Fos
• 刊名：Life Sciences
• 出版年：2016
• 出版时间：15 March 2016
• 年：2016
• 卷：149
• 期：Complete
• 页码：34-41
• 全文大小：1196 K

文摘

Evaluate the anti-hyperalgesic effect of the complex containing β-caryophyllene (βCP) and β-cyclodextrin (βCD) in a non-inflammatory chronic muscle pain mice model and investigated its action on superficial dorsal horn of the lumbar spinal cord.

d="absSec_2">Main methods

d="sp0055">The βCP-βCD complex were prepared and characterized through the DSC, TG/DTG, FTIR, XRD and SEM. The model of chronic muscle pain was induced by two injections of pH 4.0 saline (20 μL) into left gastrocnemius 5 days apart. After confirming hyperalgesia, male mice were treated with βCP-βCD (10 or 20 mg/kg; p.o.) or vehicle (saline 0.9%, p.o.) daily for 9 days. 1 h after, the mechanical hyperalgesia, muscle withdrawal thresholds and motor performance were evaluated. To evaluate the βCP-βCD action on spinal cord, animals induced with chronic muscle pain were treated with βCP-βCD (20 mg/kg; p.o.) or vehicle (saline 0.9%, p.o.) and 90 min. after, were perfused, the lumbar spinal cord collected, crioprotected, cut and submitted in an immunofluorescence protocol for Fos protein.

d="absSec_3">Key findings

d="sp0060">The characterization tests indicated that βCP were efficiently incorporated into βCD. The oral treatment with βCP-βCD, at all doses tested, produced a significant (p < 0.05) reduction on mechanical hyperalgesia and a significant (p < 0.05) increase in muscle withdrawal thresholds, without produce any alteration in force. In addition, βCP-βCD was able to significantly (p < 0.05) decrease Fos expression in the superficial dorsal horn.

d="absSec_4">Significance

d="sp0065">Thus, βCP-βCD attenuates the non-inflammatory chronic muscle pain in mice and inhibits the Fos expression in the lumbar spinal cord.