Pneumatic hydrodynamics influence transplastomic protein yields and biological responses during in vitro shoot regeneration of Nicotiana tabacum callus: Implications for bioprocess routes to plant-made biopharmaceuticals
Tissue culture is advantageous for ‘molecular farming’ in transplastomic plants. In vitro shoot regeneration of N. tabacum callus was carried out. Biological responses were correlated to pneumatic hydrodynamic parameters. Reduced growth and mitochondrial activity occurred above a critical shear rate. Transplastomic protein yield declined exponentially with increasing aeration.