A CRISPR/Cas9 mediated point mutation in the alpha 6 subunit of the nicotinic acetylcholine receptor confers resistance to spinosad in Drosophila melanogaster
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- 作者：Christoph T. Zimmera ; b ; 1 ; c.t.zimmer@exeter.ac.uk" class="auth_mail" title="E-mail the corresponding author ; William T. Garrooda ; A. Mirel Puineana ; Manuela Eckel-Zimmera ; b ; 1 ; Martin S. Williamsona ; T.G. Emyr Daviesa ; Chris Bassa ; b ; 1
- 关键词：CRISPR ; Nicotinic acetylcholine receptor ; nAChR ; Spinosad ; Insecticide resistance
- 刊名：Insect Biochemistry and Molecular Biology
- 出版年：2016
- 出版时间：June 2016
- 年：2016
- 卷：73
- 期：Complete
- 页码：62-69
- 全文大小：2096 K
文摘
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The nAChR alpha6 subunit of Drosophila melanogaster has been successfully mutated in vivo by CRISPR/Cas9 genome editing.
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We demonstrate that the G275E mutation in Dα6 confers significant resistance to spinosad.
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G275E in Dα6 resembles the phenotype reported for this particular mutation in Frankliniella occidentalis.