Co-positivity of anti-dengue virus and anti-Japanese encephalitis virus IgM in endemic area: co-infection or cross reactivity?

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• 作者：Kaleshwar Prasad Singh ; Gitika Mishra ; Parul Jain ; Nidhi P ; ey ; Rachna Nagar ; Shikha Gupta ; Shantanu Prakash ; Om Prakash ; Danish Nasar Khan ; Sakshi Shrivastav ; Desh Deepak Singh ; Amita Jain
• 关键词：Co-infection ; Co-circulation ; Dengue virus ; IgM ; Japanese encephalitis virus ; Reverse transcriptase polymerase chain reaction
• 刊名：Asian Pacific Journal of Tropical Medicine
• 出版年：February, 2014
• 年：2014
• 卷：7
• 期：2
• 页码：124-129
• 全文大小：450 K

文摘

Objective

To report high co-positivity of anti-dengue virus (DV) and anti-Japanese encephalitis virus (JEV) IgM in an area endemic for both the viruses and to discuss the possibilities of co-infection.

Methods

Serum samples from the patients who presented with fever, suspected central nervous system infection and thrombocytopenia, were tested for anti-DV IgM and anti-JEV IgM antibodies. Conventional reverse transcriptase polymerase chain reaction was done for detection of DV RNA and JEV RNA.

Results

Of 1 410 patient sera tested for anti-DV and anti-JEV antibodies, 129 (9.14%) were co-positive for both. This co-positivity was observed only in those months when anti-JEV IgM positivity was high. Titers of both anti-DV IgM and anti-JEV IgM were high in most of the co-positive cases. Among these 129 co-positive cases, 76 were tested by conventional reverse transcriptase polymerase chain reaction for both flaviviruses, of which eight cases were co-positive for DV and JEV.

Conclusions

Co-infection with more than one flavivirus species can occur in hyperendemic areas.