Analysis of pCERC7, a small antibiotic resistance plasmid from a commensal ST131 Escherichia coli, defines a diverse group of plasmids that include various segments adjacent to a multimer resolution site and encode the same NikA relaxase accessory protein enabling mobilisation

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• 作者：Robert A. Moran ; Ruth M. Hall ; ^{ruth.hall@sydney.edu.au}
• 关键词：E. coli ST131 ; Relaxase-in trans ; Mobilisation ; Antibiotic resistance
• 刊名：Plasmid
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：89
• 期：Complete
• 页码：42-48
• 全文大小：1154 K
• 卷排序：89

文摘

pCERC7, a 9712 bp ampicillin resistance plasmid, from a commensal ST131 Escherichia coli carries Tn2::IS4. Plasmids with the pCERC7 backbone or with the same Tn2 insertion have been found globally since the 1970s. pCERC7 contains an oriT and encodes a NikA accessory protein related to those of I-complex plasmids, but no NikB relaxase. Further plasmids have closely related nikA-oriT and nmr resolution sites but nine different associated regions. Replacement of the accessory modules of plasmids in this family is likely mediated by XerC/D recombination at the nmr site.