Mechanisms of resistance to fluconazole in Candida albicans clinical isolates from Iranian HIV-infected patients with oropharyngeal candidiasis

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• 作者：S. Salari^a ; ^b ; ^{sa_salari@kmu.ac.ir" class="auth_mail" title="E-mail the corresponding author} ; A.R. Khosravi^c ; S.A.A. Mousavi^a ; G.H. Nikbakht-Brojeni^d
• 关键词：Candida albicans ; Azole resistance ; Fluconazole ; Efflux pumps
• 刊名：Journal de Mycologie Médicale / Journal of Medical Mycology
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：26
• 期：1
• 页码：35-41
• 全文大小：617 K

文摘

The opportunistic pathogen Candida albicans is the major agent of oropharyngeal candidiasis (OPC) in HIV/AIDS patients. The increased use of fluconazole can lead to the emergence of azole-resistant strains and treatment failures in PLWH (people living with HIV) receiving long-term therapy for OPC. The purpose of this study was to evaluate CDR1, CDR2, MDR1, and ERG11 gene expression in C. albicans clinically isolated from HIV-infected patients in Iran.

Patients and Methods

In this study, we evaluated the molecular mechanisms of azole resistance in 20 fluconazole-resistant C. albicans isolates obtained from Iranian HIV-infected patients with oropharyngeal candidiasis by Real-Time polymerase chain reaction.

Results

The overexpression of drug efflux pump CDR1 gene was found to be the major resistance mechanism observed in these isolates. The overexpression of the CDR1 gene correlated strongly with increasing resistance to fluconazole (P < 0.05). Additionally, an increased level of mRNA in ERG11 was not observed in any of the tested isolates.

Conclusions

Our findings suggested that the CDR1 gene expression to fluconazole resistance in C. albicans is greater than other known genes.