Immunohistochemical tracking of an immune response in mammary Paget’s disease

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• 作者：Thomas Brunhuber ; Johannes Haybaeck ; Georg Schä ; fer ; Gregor Mikuz ; Eric Langhoff ; Sem Sael ; Serge Lebecque ; Nikolaus Romani ; Peter Obrist
• 关键词：Paget disease ; Immunohistochemistry ; Dendritic cells ; Breast ; Immune response
• 刊名：Cancer Letters
• 出版年：2008
• 出版时间：18 December 2008
• 年：2008
• 卷：272
• 期：2
• 页码：206-220
• 全文大小：1457 K

文摘

Dendritic cells (DC) are the most potent antigen-presenting cells of the organism. They are specialized to capture, process, and present antigen via the MHC class II as well as the MHC class I pathways to CD4⁺ and CD8⁺ T cells, respectively. This results in T cell-mediated immune responses that are likely to counteract the generation and propagation of tumors in vivo. Therefore, we studied the distribution of dendritic cells in mammary Paget’s disease.

Paraffin-embedded samples of Paget’s disease of the breast (n = 27) and of disease-free epidermis of the nipple (n = 10) were investigated immunohistochemically for the presence of dendritic cells, in particular of Langerhans cells, using antibodies against S-100, CD1a, and HLA-DR, as well as novel reagents against Langerin/CD207, DC-LAMP/CD208 and p55 (Fascin), the latter two being specific for mature dendritic cells.

Paget samples presented a decrease of CD1a⁺, S-100⁺, and Langerin⁺ intraepidermal Langerhans cells in almost all cases. This was paralleled by a concentration of immature dendritic cells in the tumor-infiltrated tissue itself. Similar to infiltrating breast carcinoma we observed a marked increase of DC-LAMP⁺ and p55⁺ mature dendritic cells in the corial tissue beneath the tumor. These cells were almost always found in ribbon-like or nodular lymphocytic infiltrates. Moreover, rare mature dendritic cells were also found in the Paget cell-infiltrated epidermis of the nipple, i.e. in the tumorous lesion itself.

These findings may indicate an effective ongoing anti-tumor immune response in this part of spreading breast cancer.