The IsoStretcher: An isotropic cell stretch device to study mechanical biosensor pathways in living cells

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• 作者：S Schü ; rmann^a ; S Wagner^a ; ^b ; S Herlitze^a ; C Fischer^a ; S Gumbrecht^a ; A Wirth-Hü ; cking^a ; G Prö ; lß ; ^a ; LA Lautscham^b ; B Fabry^b ; WH Goldmann^b ; V Nikolova-Krstevski^c ; B Martinac^d ; ^e ; O Friedrich^a ; ^{oliver.friedrich@mbt.uni-erlangen.de" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Mechanobiology ; Cell stretch device ; Mechanosensor ; Elastomer membrane ; Isotropic stretch ; Cell stiffness
• 刊名：Biosensors and Bioelectronics
• 出版年：2016
• 出版时间：15 July 2016
• 年：2016
• 卷：81
• 期：Complete
• 页码：363-372
• 全文大小：2076 K

文摘

<dl class="listitem" id="list_li0005"><dt class="label">•dt><dd>

d="p0005">A novel low-cost isotropic cell stretch system was engineered for live-cell imaging.

dd><dt class="label">•dt><dd>

d="p0010">Conical design of PDMS elastomeric membranes allow minimum z-shift during stretch.

dd><dt class="label">•dt><dd>

d="p0015">PDMS membrane-to-cell-area-stretch-transmission was explicitly calibrated in HeLa cells.

dd><dt class="label">•dt><dd>

d="p0020">Isotropic stretch of cells overexpressing nuclear lamin-A suggests global cell stiffening.

dd><dt class="label">•dt><dd>

d="p0025">Isotropic stretch activates Ca²⁺ entry in atrial HL-1 myocytes.

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