Frequency of HPRT mutants in humans exposed to vinyl chloride via an environmental accident
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- 作者：Becker ; Roswitha ; Nikolova ; Teodora ; Wolff ; Ilona ; Lovell ; David ; et. al.
- 关键词：Accidental environmental exposure ; HPRT-gene ; Human T-lymphocytes ; In vivo ; Mutant frequency ; Vinyl chloride ; HPRT ; hypoxanthine-guanine-phosphoribosyl-transferase ; VCM ; vinyl chloride monomer ; PVC ; polyvinyl chloride ; TG ; 6-thioguanine ; MF ; mutant freque
- 刊名：Mutation Research/Genetic Toxicology and Environmental Mutagenesis
- 出版年：2001
- 出版时间：July 25, 2001
- 年：2001
- 卷：494
- 期：1-2
- 页码：87-96
- 全文大小：137 K
文摘
The mutant frequency (MF) in the hypoxanthine-guanine-phosphoribosyl-transferase (HPRT) locus of peripheral blood T-lymphocytes was measured in a population environmentally exposed to vinyl chloride — a toxic and carcinogenic substance through an accidental release into the atmosphere. It was compared to MF in a control group of unexposed individuals. Both groups were re-investigated in a follow-up study, 2 years later. No significant difference could be observed in MF between exposed and controls either at the accident nor in the follow-up study. Approximately the same mean HPRT mutant frequencies were observed for both groups in T-lymphocytes from blood samples obtained shortly after the accident and from the follow-up blood samples. Both groups showed a higher mean MF in the re-investigation samples which is most probably due to the significantly lower average cloning efficiency (CE) under non-selective conditions and because of the inverse relationship between CE and MF. The exposed population showed a higher mean T-cell CE at the initial blood sampling as compared to the control group. The concurrent cytogenetic analyses of peripheral lymphocytes showed a significant increase in cells with aberrations in the exposed population. Clastogenic but not mutagenic activity of vinyl chloride was observed in our study.