Light-up and FRET aptamer reporters; evaluating their applications for imaging transcription in eukaryotic cells
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- 作者：Muslum Ilgua ; 1 ; Judhajeet Raya ; 2 ; Lee Bendicksona ; c ; Tianjiao Wangc ; 3 ; Ivan M. Geraskinb ; George A. Krausb ; c ; Marit Nilsen-Hamiltona ; c ; marit@iastate.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：ddH2O ; deionized distilled water ; MGA ; malachite green aptamer ; MG ; malachite green
- 刊名：Methods
- 出版年：2016
- 出版时间：1 April 2016
- 年：2016
- 卷：98
- 期：Complete
- 页码：26-33
- 全文大小：1529 K
文摘
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A new Spinach ligand with 40-fold higher affinity and 4-fold higher fluorescence.
High background adsorption with light-up aptamer ligands.
FRET-based IMAGEtags signal RNA expression in the presence of high backgrounds.
Tandem arrays of light-up aptamers do not cooperate for increased binding affinity.