Neutral monosaccharide composition analysis of plant-derived oligo- and polysaccharides by high performance liquid chromatography
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- 作者：Jun Yan ; Songshan Shi ; Hongwei Wang ; Ruimin Liu ; Ning Li ; Yonglin Chen ; Shunchun Wang ; shunchunwang@126.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Monosaccharide composition ; Carbohydrates ; Hydrophilic interaction liquid chromatography ; Charged aerosol detector ; Eclipta prostrasta L.
- 刊名：Carbohydrate Polymers
- 出版年：2016
- 出版时间：20 January 2016
- 年：2016
- 卷：136
- 期：Complete
- 页码：1273-1280
- 全文大小：1226 K
文摘
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A simple and fast HILIC-CAD method was developed for monosaccharides profiling.
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Effects of column type, additives, temperature and pH were studied for optimal conditions.
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Validation of the method showed good linearity, accuracy, precision and sensitivity.
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Successfully applied to monosaccharide analysis of stachyose and EAPW.
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First report on the simultaneous and direct determination of the eight sugars by HPLC.