Specific inhibition of cyclin-dependent kinase 5 activity induces motor neuron development in vivo

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作者：Jyotshnabala Kanungo ; Ya-Li Zheng ; Niranjana D. Amin ; Sukhbir Kaur ; Ramani Ramch ; ran ; Harish C. Pant
关键词：Motor neuron ; Neurogenesis ; Protein kinase ; Morpholino knockdown ; Zebrafish
刊名：Biochemical and Biophysical Research Communications
出版年：2009
出版时间：14 August 2009
年：2009
卷：386
期：1
页码：263-267
全文大小：551 K

文摘

Cyclin-dependent kinase 5 (cdk5) is a ubiquitous protein activated by specific activators, p35 and p39. Cdk5 regulates neuronal migration, differentiation, axonogenesis, synaptic transmission and apoptosis. However, its role in motor neuron development remains unexplored. Here, using gain and loss-of-function analyses in developing zebrafish embryos, we report that cdk5 plays a critical role in spinal and cranial motor neuron development. Cdk5 knockdown results in supernumerary spinal and cranial motor neurons. While a dominant negative, kinase-dead cdk5 promotes the generation of supernumerary motor neurons; over-expression of cdk5 suppresses motor neuron development. Thus, modulating cdk5 activity seems promising in inducing motor neuron development in vivo.

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