Effects of select nitrocompounds on in vitro
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- 作者：Robin C. Anderson ; Nathan A. Krueger ; Thaddeus B. Stanton ; Todd R. Callaway ; Thomas S. Edrington ; Roger B. Harvey ; Yong Soo Jung ; David J. Nisbet
- 关键词：Formate dehydrogenase/formate hydrogen lyase ; Hydrogenase ; Methane production ; Rumen
- 刊名：Bioresource Technology
- 出版年：2008
- 出版时间：December 2008
- 年：2008
- 卷：99
- 期：18
- 页码：8655-8661
- 全文大小：330 K
文摘
Ruminal methane (CH4) production results in the loss of up to 12 % of gross energy intake and contributes nearly 20 % of the United States’ annual emission of this greenhouse gas. We report the effects of select nitrocompounds on ruminal fermentation after 22 h in vitro incubation (39 °C) with or without additions of hydrogen (H2), formate or both. In incubations containing no added reductant, CH4 production was inhibited 41 % by 2-nitro-1-propanol (2NPOH) and >97 % by 3-nitro-1-propionic acid (3NPA), nitroethane (NE) and 2-nitroethanol (2NEOH) compared to non-treated controls and H2 did not accumulate. With formate as the sole added reductant, nitro-treatment reduced CH4 production by >99 % and caused 42 % to complete inhibition of formate catabolism compared to controls, and the accumulation of H2 increased slightly. Nitro-treatment decreased CH4 production 57–98 % from that of controls when supplied H2 or formate plus H2. Formate catabolism was decreased 42–84 % from that in controls by all nitro-treatments except 3NPA with both formate and H2. Greater than 97 % of the added H2 was catabolized within controls; >84 % was catabolized in nitro-treated incubations. Acetate, propionate and butyrate accumulations were unaffected by nitro-treatment irregardless of reductant; however, effects on ammonia and branched chain fatty acid accumulations varied. These results suggest that nitro-treatment inhibited formate dehydrogenase/formate hydrogen lyase and hydrogenase activity.