Profiling gibberellin (GA₃)-responsive genes in mature mandarin fruit using a citrus 22K oligoarray

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• 作者：Hiroshi Fujii ; Takehiko Shimada ; Aiko Sugiyama ; Tomoko Endo ; Fumie Nishikawa ; Michiharu Nakano ; Yoshinori Ikoma ; Tokuro Shimizu ; Mitsuo Omura
• 关键词：Microarray ; Citrus ; Gibberellin ; Non-climacteric ; Ripening
• 刊名：Scientia Horticulturae
• 出版年：2008
• 出版时间：1 May 2008
• 年：2008
• 卷：116
• 期：3
• 页码：291-298
• 全文大小：537 K

文摘

Gibberellin₃ (GA₃)-responsive genes were investigated with a citrus 22K oligoarray¹ to further the understanding of transcriptional regulation by GA₃ treatment in Satsuma mandarin fruit (Citrus unshiu Marc.). 213 GA₃-responsive genes were identified that showed a 3-fold or greater expression change after 72 h GA₃ treatment, compared to expression after 72 h air treatment. GA₃ treatment induced expression of pathogenesis-related (PR) proteins and genes that function in photosynthesis, chloroplast biogenesis, resistance, defense and stress. Also, GA₃ treatment reduced the transcription of several ethylene-inducible genes, such as carotenoid metabolic genes, which are associated with fruit ripening.