Inflammatory cytokines induce a reduction in E-cadherin expression and morphological changes in MDCK cells

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• 作者：Tomochika Saito ; Kota Yoshida ; Kaori Matsumoto ; Kohei Saeki ; Yuiko Tanaka ; Siew-Mei Ong ; Nobuo Sasaki ; Ryohei Nishimura ; Takayuki Nakagawa ; ^{anakaga@mail.ecc.u-tokyo.ac.jp" class="auth_mail}
• 关键词：Epithelial&ndash ; mesenchymal transition ; Madin-Darby canine kidney cell ; Transforming growth factor beta ; Tumor necrosis factor alpha ; Interleukin 6 ; Inflammation
• 刊名：Research in Veterinary Science
• 出版年：April, 2014
• 年：2014
• 卷：96
• 期：2
• 页码：288-291
• 全文大小：941 K

文摘

Epithelial-mesenchymal transition (EMT) is a fundamental phenomenon in organisms that occurs during gastrulation, wound healing, and cancer metastasis. Various cytokines induce EMT processes through complex mechanisms. Inflammatory cytokines, such as tumor necrosis factor alpha (TNF-伪) and interleukin 6 (IL-6), induce EMT in human cell lines. However, whether inflammatory cytokines can affect EMT processes in canine cell lines remains unclear. In this study, we investigated the role of transforming growth factor beta (TGF-尾), TNF-伪, and IL-6 in Madin-Darby canine kidney (MDCK) cells. We found that the localization of E-cadherin, a cell adhesion molecule, was shifted and that its expression was decreased. We also observed morphological changes in MDCK cells under persistent stimulation of inflammatory cytokines. Morphological changes in cells may occur during late stages of EMT processes; inflammatory cytokines may be important in these changes.