Analysis of adaptive mutations selected during the consecutive passages of hepatitis E virus produced from an infectious cDNA clone
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- 作者：Shigeo Nagashima ; Tominari Kobayashi ; Toshinori Tanaka ; Tanggis ; Suljid Jirintai ; Masaharu Takahashi ; Tsutomu Nishizawa ; Hiroaki Okamoto ; hokamoto@jichi.ac.jp" class="auth_mail" title="E-mail the corresponding author
- 关键词：Adaptation ; Cell culture ; Hepatitis E virus ; Infectious cDNA clone ; Reverse genetics system ; Synonymous mutation
- 刊名：Virus Research
- 出版年：2016
- 出版时间：2 September 2016
- 年：2016
- 卷：223
- 期：Complete
- 页码：170-180
- 全文大小：1512 K
文摘
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A cloned genotype 3 HEV (pJE03-1760F/wt) was passaged 10 times in A549 cells.
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The Passage 10 virus had two non-synonymous and four synonymous mutations.
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Full-length cDNA clones with a single, two, or all six mutations were constructed.
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HEV with all six mutations produced 2 logs more virions than did starting virus.
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Both non-synonymous and synonymous HEV mutations enhanced virus production.