A validated liquid chromatographic method to determine protein-bound tyrosine oxidation, nitration and chlorination by-products.
Plasma, Liver and Brain samples of hypoxic and normoxic animals from a postnatal hypoxia newborn piglets model.
Small sample volume and suitable sample pre-treatment that includes protein extraction and subsequent enzymatic hydrolysis.
High-throughput of sample analysis and high selectivity for Phe, p-Tyr, o-Tyr, m-Tyr, 3Cl-Tyr, 3NO2-Tyr phenylalanine, p-tyrosine, ortho-tyrosine, meta-tyrosine, 3-nitrotyrosine and 3-chlorotyrosine.