Enzymatic synthesis of 3-hydroxypropionic acid at high productivity by using free or immobilized cells of recombinant Escherichia coli
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- 作者：Shanshan Yua ; b ; 1 ; Peiyuan Yaob ; 1 ; Jianjiong Lib ; Jie Renb ; Jing Yuanb ; Jinhui Fengb ; Min Wanga ; Qiaqing Wub ; wu_qq@tib.cas.cn" class="auth_mail" title="E-mail the corresponding author ; Dunming Zhub ; zhu_dm@tib.cas.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Nitrilase ; Immobilization ; 3-Hydroxypropionic acid ; Hydrolysis ; Enzyme catalysis
- 刊名：Journal of Molecular Catalysis B: Enzymatic
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：129
- 期：Complete
- 页码：37-42
- 全文大小：1415 K
文摘
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A highly substrate-tolerant nitrilase was discovered for the synthesis of 3-hydroxypropionic acid.
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The immobilized cells catalyzed the complete hydrolysis of up to 7.0 mol/L of 3-hydroxypropionitrile.
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The immobilized cells showed no activity loss up to 30 batches in distilled water.
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High titer (184.7 g/L) and productivity (36.9 g/(L h)) were obtained.
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A feasible process was developed for the industrial production of 3-hydroxypropionic acid.