Biotransformation of nitroaromatics and their effects on mixed function oxidase system

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• 作者：I. Soojhawon ; P.D. Lokhande ; K.M. Kodam and K.R. Gawai
• 关键词：Mixed function oxidase system ; Biotransformation ; Acinetobacter junii A8 ; Nitroaromatics ; Cytochrome P-450
• 刊名：Enzyme and Microbial Technology
• 出版年：2005
• 出版时间：2005
• 年：2005
• 卷：37
• 期：5
• 页码：527-533
• 全文大小：150 K

文摘

Acinetobacter junii A8 was able to biotransform nitroaromatic compounds like o-nitroaniline, o-nitrotoluene, m-nitrotoluene, 2,4,6-trinitrotoluene and o-nitrophenol under aerobic condition, within 36 h of incubation. These compounds, which are normally considered to be toxic to microorganisms, caused the induction of drug metabolizing enzymes such as cytochrome P-450, aminopyrine N-demethylase, acetanilide hydroxylase and glutathione S-transferase. These enzymes constitute the bacterial mixed function oxidase system. Induction of this system implies its involvement in biotransformation of these nitro compounds. The biotransformed metabolites were characterized by IR, ¹H NMR and GC–mass spectroscopy. It was observed that o-nitroaniline and m-nitrotoluene were reduced to their respective amines, whereas o-nitrotoluene was oxidized to acid. However, 2,4,6-trinitrotoluene (TNT) and o-nitrophenol were completely mineralized. The metabolites of TNT biotransformation were characterized as 2,6-dinitro-4-nitrosotoluene, 4-amino-2,6-dinitrotoluene and 4-amino-2,6-dinitrobenzoic acid. The result of this study showed that these nitroaromatics induced the mixed function oxidase system, which resulted in the degradation of these compounds.