Reduction of nitro phenols using nitroreductase from E. coli
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- 作者：Rongji Dai ; Jie Chen ; Jie Lin ; Shenyuan Xiao ; Shi Chen ; Yulin Deng
- 关键词：Nitroreductase ; Nitrophenols ; Reduction
- 刊名：Journal of Hazardous Materials
- 出版年：2009
- 出版时间：15 October 2009
- 年：2009
- 卷：170
- 期：1
- 页码：141-143
- 全文大小：274 K
文摘
The reductions of nitrophenols catalyzed by nitroreductase from E. coli in the presence of NADH were investigated in this paper. 4-Aminophenol and 4-hydroxylaminophenol were found in the reductive products of 4-nitrophenol and the maximum reductive ratio was about 83.49 % when the reaction time was 70 min; 4,6-dinitro-2-pimelie kelone was found in the reductive products of 2,4-dinitrophenol and the maximum reductive ratio was about 75.28 % when the reaction time was 80 min; 2,4-dinitrophenol and 4,6-dinitro-2-pimelie kelone were found in the reductive products of 2,4,6-trinitrophenol and the maximum reductive ratio was about 62.08 % when the reaction time was 100 min. The similar reductive ratios of nitrophenols were obtained under aerobic and anaerobic conditions. The results indicated that nitroreductase was an oxygen-insensitive enzyme.