Simultaneous determination of four tobacco-specific N

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• 作者：Dominique Kavvadias ; Gerhard Scherer ; Michael Urban ; Francis Cheung ; Graham Errington ; Jim Shepperd ; Mike McEwan
• 关键词：Tobacco-specific N-nitrosamines (TSNA) ; 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) ; 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) ; N-nitrosonornicotine (NNN) ; N-nitrosoanabasine (NAB) ; N-nitrosoanatabine (NAT)
• 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
• 出版年：2009
• 出版时间：15 April 2009
• 年：2009
• 卷：877
• 期：11-12
• 页码：1185-1192
• 全文大小：660 K

文摘

Tobacco-specific N-nitrosamines (TSNA) include 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), N-nitrosonornicotine (NNN), N-nitrosoanabasine (NAB) and N-nitrosoanatabine (NAT). TSNA are suggested to play an important role in tobacco smoke carcinogenesis. We have developed and validated an LC–MS/MS method for the determination of total (free and conjugated) TSNA in human urine. The limits of detection (LOD) were 2.0, 0.8, 1.1 and 0.7 pg/ml for NNAL, NNN, NAB and NAT, respectively. Smokers were found to have significantly higher levels of TSNA in their urine than nonsmokers. In conclusion, the newly developed method is suitable for assessing the tobacco use-related exposure to NNK, NNN, NAB and NAT.