Identification and functional evaluation of Leishmania braziliensis Nicotinamide Mononucleotide Adenylyltransferase
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- 作者：Luis E. Contreras ; Rafik Neme1 ; Marí ; a H. Ramí ; rez ; mhramirezh@unal.edu.co" class="auth_mail" title="E-mail the corresponding author
- 关键词：Leishmania ; Nicotinamide adenine dinucleotide (NAD+) ; Nicotinamide Mononucleotide Adenylyltransferase (NMNAT)
- 刊名：Protein Expression and Purification
- 出版年：2015
- 出版时间：November 2015
- 年：2015
- 卷：115
- 期：Complete
- 页码：26-33
- 全文大小：2098 K
文摘
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The NMNAT enzymes of different Leishmania species were identified.
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The enzymatic activity of L. braziliensis NMNAT was confirmed.
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Leishmania NMNAT contains a specific amino-terminal insertion.
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Deletion of the insertion is negatively correlated with in vitro enzymatic activity.
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We suggest the amino-terminal insertion as a promising pharmacological target.