STED microscopy for nanoscale imaging in living brain slices
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- 作者：Ronan Ché ; reau1 ; Jan Tø ; nnesen ; U. Valentin Nä ; gerl ; valentin.nagerl@u-bordeaux.fr" class="auth_mail" title="E-mail the corresponding author
- 关键词：Superresolution microscopy ; STED microscopy ; Brain slices ; Neurons ; Dendritic spines ; Axons
- 刊名：Methods
- 出版年：2015
- 出版时间：15 October 2015
- 年：2015
- 卷：88
- 期：Complete
- 页码：57-66
- 全文大小：1893 K
文摘
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The optical concepts of superresolution microscopy are explained.
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The design and construction of a home-built STED microscope is explained.
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The performance & testing of a STED microscope is described.
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Recent technical developments to improve & broaden the scope of STED microscopy are outlined.
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Examples of neurobiological applications for live-cell imaging in brain slices are presented.