Ginsenoside Rb1 protects against damage to the spiral ganglion cells after cochlear ischemia
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- 作者：Kensuke Fujita ; Nobuhiro Hakuba ; Ryuji Hata ; Isao Morizane ; Tadashi Yoshida ; Masachika Shudou ; Masahiro Sakanaka ; Kiyofumi Gyo
- 关键词：Transient cochlear ischemia ; Spiral ganglion cells ; Ginsenoside Rb1 ; TUNEL staining ; Bcl-xL protein
- 刊名：Neuroscience Letters
- 出版年：2007
- 出版时间：26 March 2007
- 年：2007
- 卷：415
- 期：2
- 页码：113-117
- 全文大小：993 K
文摘
The effects of transient cochlear ischemia on spiral ganglion cells (SGCs) were studied in Mongolian gerbils. Ischemic insult was induced by occluding the bilateral vertebral arteries of gerbils for 15 min. Seven days after ischemia, the percentage of SGCs decreased to 67.5 % from the preischemic baseline in the basal turn. Evaluation with immunohistochemical staining showed TUNEL-positive reactions in the SGCs with fragmented nuclei. In addition, we investigated the protective effects of ginsenoside Rb1 (gRb1) against ischemic injury to SGCs. Seven days after ischemia, the auditory brainstem response threshold shift was significantly reduced and the percentage of SGCs decreased to 90.2 % from the preischemic baseline in the basal turn in the gRb1-treated group. These findings suggest that gRb1 prevented hearing loss caused by ischemic injury to SGCs in Mongolian gerbils.