Editorial on “Peak capacity in unidimensional chromatography” by U.D. Neue

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• 作者：Nobuo Tanaka
• 关键词：Capillary zone electrophoresis ; Surfactants ; Adsorbed polymers ; SMIL ; Protein adsorption ; Protein recovery ; Efficiency ; Reproducibility
• 刊名：Journal of Chromatography A
• 出版年：2008
• 出版时间：14 March 2008
• 年：2008
• 卷：1184
• 期：1-2
• 页码：106
• 全文大小：50 K

文摘

Adsorption of proteins, particularly basic proteins onto fused silica capillaries severely degrades capillary electrophoretic performance. This review provides a synopsis of the fundamentals underlying protein adsorption and its impact on CE performance. The efficacy of small molecule background electrolyte additives, surfactants, physically adsorbed polymers (dynamic and static), and successive multiple ionic-polymer layer coatings are evaluated using a number of performance metrics. Peak efficiency and migration time reproducibility are used as measures of reversible protein adsorption, while protein recovery, electroosmotic flow reproducibility and step changes in the baseline are used as indicators of irreversible protein adsorption.