Single base oligodeoxyguanosine upregulates Fas ligand release by nonspecific cytotoxic cells

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• 作者：Kaur ; Harjeet ; Jaso-Friedmann ; Liliana ; Evans ; Donald L.
• 关键词：Nonspecific cytotoxic cells ; Single base oligos ; Oligodeoxyguanosine ; Soluble Fas ligand ; Fas receptor ; Programmed cell death ; Annexin-V ; DNA hypoploidy ; NCC ; nonspecific cytotoxic cells ; NCCRP-1 ; nonspecific cytotoxic cells receptor protein-1 ; FasL ; Fas
• 刊名：Developmental and Comparative Immunology
• 出版年：2003
• 出版时间：May 17, 2003
• 年：2003
• 卷：28
• 期：6
• 页码：571-579
• 全文大小：173 K

文摘

Nonspecific cytotoxic cells (NCC) are a type of teleost NK-like cell. In the present study a novel stimulus secretion model is described for catfish NCC utilizing single base oligodeoxyguanosine. Binding of guanosine 20-mers (dG20) to NCC up-regulated expression of cytosolic FasL detected by an anti-human FasL monoclonal antibody (mab). In vitro treatment of purified NCC with dG20 produced a 7-fold increase in expression of soluble Fas ligand (sFasL) after 3 h. Antibody binding to NCC was saturable and approximately 30–35 % of total NCC were positive for sFasL expression. The teleost FasL equivalent produced programmed cell death of appropriate FasR positive targets. Supernatants from dG20 activated NCC produced hypoploidy and annexin-V binding by FasR bearing HL-60 cells. Treatment of activated supernatants with immobilized anti-FasL mab neutralized these activities. These studies demonstrated that an NK like cell (NCC) produces and secretes sFasL following binding by single base oligodeoxyguanosine.