Rcf1 Mediates Cytochrome Oxidase Assembly and Respirasome Formation, Revealing Heterogeneity of the Enzyme Complex

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• 作者：Milena Vukotic¹ ; ⁴ ; Silke Oeljeklaus⁵ ; ⁷ ; Sebastian Wiese⁵ ; ⁷ ; F.  ; Nora V&#246 ; gtle⁴ ; Chris Meisinger⁴ ; ⁷ ; Helmut  ; E. Meyer⁸ ; Anke Zieseniss² ; Doerthe  ; M. Katschinski² ; Daniel  ; C. Jans⁹ ; Stefan Jakobs³ ; ⁹ ; Bettina Warscheid⁵ ; ⁶ ; ⁷ ; Peter Rehling¹ ; ¹⁰ ; ^{peter.rehling@medizin.uni-goettingen.de} ; Markus Deckers¹
• 刊名：Cell Metabolism
• 出版年：2012
• 出版时间：7 March, 2012
• 年：2012
• 卷：15
• 期：3
• 页码：336-347
• 全文大小：1570 K

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Summary

The terminal enzyme of the mitochondrial respiratory chain, cytochrome oxidase, transfers electrons to molecular oxygen, generating water. Within the inner?mitochondrial membrane, cytochrome oxidase assembles into supercomplexes, together with other respiratory chain complexes, forming so-called respirasomes. Little is known about how these higher oligomeric structures are attained. Here we report on Rcf1 and Rcf2 as cytochrome oxidase subunits in S.?cerevisiae. While Rcf2 is specific to yeast, Rcf1 is a conserved subunit with two human orthologs, RCF1a and RCF1b. Rcf1 is required for growth in hypoxia and complex assembly of subunits Cox13 and Rcf2, as well as for the oligomerization of?a subclass of cytochrome oxidase complexes into respirasomes. Our analyses reveal that the cytochrome oxidase of mitochondria displays intrinsic heterogeneity with regard to its subunit composition and that distinct forms of respirasomes can be formed by complex variants.