Selective whole-cell biosynthesis of the designer drug metabolites 15- or 16-betahydroxynorethisterone by engineered Cytochrome P450 BM3 mutants
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- 作者：Jelle Reinena ; Galvin Vredenburga ; Karoline Klaeringa ; Nico P.E. Vermeulena ; Jan N.M. Commandeura ; Maarten Honingb ; J. Chris Vosa ; j.c.vos@vu.nl" class="auth_mail" title="E-mail the corresponding author
- 关键词：Whole-cell biocatalysis ; Cytochrome P450 BM3 ; Norethisterone ; Stereo- and regioselectivity ; Biotechnology
- 刊名：Journal of Molecular Catalysis B: Enzymatic
- 出版年：2015
- 出版时间：November 2015
- 年：2015
- 卷：121
- 期：Complete
- 页码：64-74
- 全文大小：1378 K
文摘
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CYP BM3 was used as a tool for whole-cell biotransformation of valuable drug metabolites.
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CYP BM3 mutants regioselectively convert NET into 15尾- or 16尾-OH-NET.
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E. coli is a better host organism than S. cerevisiae for in vivo NET bioconversion.
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Resting cells produce higher amounts of NET metabolites than growing cells.
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A large-scale fermentor setup for production of 15尾- and 16尾-OH-NET was developed.